Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/26859
Title: Ultrasound-induced physiological effects and secondary metabolite (saponin) production in Panax ginseng cell cultures
Authors: Lin, L
Wu, J 
Ho, KP
Qi, S
Keywords: Panax ginseng
Plant cell culture
Saponin
Secondary metabolites
Ultrasound
Issue Date: 2001
Publisher: Elsevier
Source: Ultrasound in medicine and biology, 2001, v. 27, no. 8, p. 1147-1152 How to cite?
Journal: Ultrasound in medicine and biology 
Abstract: This work was aimed at the effects of ultrasound (US) on the growth and secondary metabolite biosynthesis of cultured plant cells. Suspension cultures of Panax ginseng cells were exposed to US at power density below 82 mW/cm3 for short periods of time (1-4 min) in a US bath (38.5-kHz fixed frequency and 810 W maximum peak power). Under most exposure conditions, US stimulated the biosynthesis of secondary metabolites, the ginsenoside saponins of ginseng cells, increasing the total saponin content of the cell by up to 75%. The growth and viability of ginseng cells were usually depressed immediately after the exposure to US, but recovered gradually to levels similar to those of a normal culture in a few days, with virtually no net loss of biomass yield at the end of the culture period. At some lower US doses, sonicated cultures could even reach slightly higher biomass yields than that of normal cultures. The effects of US on cell growth and secondary metabolite yield showed a significant correlation with the total US energy emitted (i.e., the product of US power and exposure time). Mechanical stress and microstreaming induced by acoustic cavitation were considered as the most possible causes of the various physiological effects of US on ginseng cells. In particular, the stimulation of secondary metabolite production by US may be a result of US-induced plant cell defense response.
URI: http://hdl.handle.net/10397/26859
ISSN: 0301-5629
EISSN: 1879-291X
DOI: 10.1016/S0301-5629(01)00412-4
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