Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/26505
Title: Impedance sensing of DNA immobilization and hybridization by microfabricated alumina nanopore membranes
Authors: Wu, S
Ye, W
Yang, M
Taghipoor, M
Meissner, R
Brugger, J
Renaud, P
Keywords: Alumina nanopore membrane
DNA sensor
Electrical impedance spectroscopy
Microfabrication
Issue Date: 2015
Publisher: Elsevier
Source: Sensors and actuators. B, Chemical, 2015, v. 216, p. 105-112 How to cite?
Journal: Sensors and actuators. B, Chemical 
Abstract: In this work we demonstrate microfabricated thin alumina nanopore membranes as a platform for impedance sensing of DNA immobilization and hybridization. We develop a wafer-scale fabrication of free-standing alumina nanopore membranes with well controlled thickness, pore diameter and overall pore density. One 1 cm × 1 cm single chip contains an array of 69 membranes. Each membrane is 100 μm × 100 μm large and 2 μm thick, with pore diameter of 120 nm. With low pore density of ∼6 pores/μm2, nanopore resistance and membrane capacitance can be recognized clearly in the electrochemical impedance spectrum from 100 to 1 MHz. The total surface area can be further increased by the coating of silica nanoparticles with ∼20 nm in diameter. During the immobilization of probe ssDNA to (3-glycidoxypropyl) trimethoxysilane functionalized surface, the nanopore resistance drops significantly by 80%, whereas the membrane capacitance increases less than 2%. After hybridization with complementary DNA, the nanopore resistance increases up to 10%. Non-complementary ssDNA has no obvious effect. The detection limit is 12.5 nM in phosphate-buffered saline (PBS) solution.
URI: http://hdl.handle.net/10397/26505
ISSN: 0925-4005
EISSN: 1873-3077
DOI: 10.1016/j.snb.2015.03.094
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