Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/25791
Title: G-Quadruplex conformational change driven by pH variation with potential application as a nanoswitch
Authors: Yan, YY
Tan, JH
Lu, YJ
Yan, SC
Wong, KY 
Li, D
Gu, LQ
Huang, ZS
Keywords: Acidic pH
Conformational interconversion
G-Quadruplex
Nanoswitch
pH-driven Thiazole orange (TO)
Issue Date: 2013
Publisher: Elsevier
Source: Biochimica et biophysica acta. General subjects, 2013, v. 1830, no. 10, p. 4935-4942 How to cite?
Journal: Biochimica et biophysica acta. General subjects 
Abstract: Background G-Quadruplex is a highly polymorphic structure, and its behavior in acidic condition has not been well studied. Methods Circular dichroism (CD) spectra were used to study the conformational change of G-quadruplex. The thermal stabilities of the G-quadruplex were measured with CD melting. Interconversion kinetics profiles were investigated by using CD kinetics. The fluorescence of the inserted 2-Aminopurine (Ap) was monitored during pH change and acrylamide quenching, indicating the status of the loop. Proton NMR was adopted to help illustrate the change of the conformation. Results G-Quadruplex of specific loop was found to be able to transform upon pH variation. The transformation was resulted from the loop rearrangement. After screening of a library of diverse G-quadruplex, a sequence exhibiting the best transformation property was found. A pH-driven nanoswitch with three gears was obtained based on this transition cycle. Conclusions Certain G-quadruplex was found to go through conformational change at low pH. Loop was the decisive factor controlling the interconversion upon pH variation. G-Quadruplex with TT central loop could be converted in a much milder condition than the one with TTA loop. It can be used to design pH-driven nanodevices such as a nanoswitch. General significance These results provide more insights into G-quadruplex polymorphism, and also contribute to the design of DNA-based nanomachines and logic gates.
URI: http://hdl.handle.net/10397/25791
ISSN: 0304-4165
EISSN: 1872-8006
DOI: 10.1016/j.bbagen.2013.06.019
Appears in Collections:Journal/Magazine Article

Access
View full-text via PolyU eLinks SFX Query
Show full item record

SCOPUSTM   
Citations

9
Last Week
0
Last month
0
Citations as of Aug 12, 2017

WEB OF SCIENCETM
Citations

9
Last Week
0
Last month
0
Citations as of Aug 12, 2017

Page view(s)

44
Last Week
3
Last month
Checked on Aug 13, 2017

Google ScholarTM

Check

Altmetric



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.