Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/25735
Title: Catalytically impaired fluorescent Class C £]-lactamase enables rapid and sensitive cephalosporin detection by stabilizing fluorescence signals : implications for biosensor design
Authors: Tsang, MW
So, PK
Liu, SY
Tsang, CW
Chan, PH 
Wong, KY 
Leung, YC 
Keywords: Cephalosporin
Fluorescein
Fluorescent biosensor
Food industry
£]-lactamase
Issue Date: 2015
Publisher: Wiley-VCH
Source: Biotechnology journal, 2015, v. 10, no. 1, p. 126-135 How to cite?
Journal: Biotechnology journal 
Abstract: Biosensors have found applications in many sectors including the food industry, where cephalosporin detection has played an important role in reducing the incidence of cephalosporin contamination, ensuring food safety, and reducing the spread of antibiotic resistance. Taking advantage of the specific interaction between £]-lactamase and its cephalosporin substrates/inhibitors, we previously constructed a biosensor based on a fluorescein-labeled class C £]-lactamase mutant, V211Cf, for specific and reagentless detection of cephalosporins and class C £]-lactamase inhibitors (Anal. Chem. 2011, 83, 1996-2004). Upon the addition of substrate/ inhibitor (i.e. the biosensor's analyte), the analyte induced a change in the local environment of the fluorescein molecule that was covalently tethered to a site close to the enzyme's active site (the 211 position), triggering a fluorescence enhancement of V211Cf. To improve the performance of V211Cf for better cephalosporin det
URI: http://hdl.handle.net/10397/25735
ISSN: 1860-6768
EISSN: 1860-7314
DOI: 10.1002/biot.201400140
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