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Title: Determination of butylidenephthalide in Ligusticum chuanxiong by HPLC
Authors: Wang, CY
Zhang, H
Qian, ZM
Keywords: Butylidenephthalide
Ligusticum chuanxiong
Issue Date: 2007
Publisher: 中國醫學會
Source: 中国中药杂志 (China journal of Chinese materia medica), 2007, v. 32, no. 6, p. 508-510 How to cite?
Journal: 中国中药杂志 (China journal of Chinese materia medica) 
Abstract: Objective; To determine butylidenephthalide in Ligusticum Chuanxiong with RP-HPLC. Method; The sample was extracted with methanol using sonication. The ESTD was used to quantify butylidenephthalide. HPLC separation was carried out in a Hypersil ODS columm (4.6 mm × 150 mm,5 μm) , eluted at 1 mL · min-1 with methanol-5% isopropyl alcohol (60:40) at 25 °C. The detection wavelength was 230 nm. Result; The linear range was 0.07-0.7 μg for butylidenephthalide. The average recovery was 95.3% , and RSD was 2.3% (n = 6). Conclusion; This method was simple and could be used to determine butylidenephthalide with satisfactory accuracy and reproducibility.
ISSN: 1001-5302
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