Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/25313
Title: Role of tyrosinase in the genoprotective effect of the edible mushroom, Agaricus bisporus
Authors: Shi, YL
Benzie, IFF 
Buswell, JA
Keywords: Agaricus bisporus
Antioxidants
Comet assay
Genoprotection
L-DOPA
Reactive oxygen species
Tyrosinase
Issue Date: 2002
Source: Life sciences, 2002, v. 70, no. 14, p. 1595-1608 How to cite?
Journal: Life Sciences 
Abstract: A heat-labile protein has been identified in fruit bodies of the edible mushroom, Agaricus bisporus, which protects Raji cells (a human lymphoma cell line) against H 2O 2-induced oxidative damage to cellular DNA. This protein has been purified following salt fractionation, combined with ion-exchange, hydrophobic interaction and adsorption chromatography. Based on catalytic and electrophoretic properties, and inhibition studies using tropolone, the protein was identified as tyrosinase. The genoprotective effect of A. bisporus tyrosinase, determined using the single-cell gel electrophoresis ("Comet") assay, has been shown to be dependent upon the enzymic hydroxylation of tyrosine to L-DOPA and subsequent conversion of this metabolite to dopaquinone. The possible role of dopaquinone, and other L-DOPA oxidation products, in enhancing the cellular antioxidant defence mechanisms is discussed.
URI: http://hdl.handle.net/10397/25313
ISSN: 0024-3205
DOI: 10.1016/S0024-3205(01)01546-6
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