Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/25305
Title: Identification of the heterogeneous nuclear ribonucleoprotein A2/B1 as the antigen for the gastrointestinal cancer specific monoclonal antibody MG7
Authors: Lee, CH
Lum, JHK
Cheung, BPY
Wong, MS 
Butt, YKC
Tam, MF
Chan, WY
Chow, C
Hui, PK
Kwok, FSL
Lo, SCL 
Fan, DM
Keywords: Cancer biomarker
Gastrointestinal cancer
Human heterogeneous nuclear ribonucleoprotein A2/B1
MG7
Issue Date: 2005
Publisher: Wiley-VCH
Source: Proteomics, 2005, v. 5, no. 4, p. 1160-1166 How to cite?
Journal: Proteomics 
Abstract: MG7 is an early gastrointestinal cancer specific monoclonal antibody. It can detect gastric cancer with high sensitivity and specificity. However, the target antigen for MG7 has not been identified. Western blot analysis revealed that the MG7 antibody reproducibly recognized two ∼35 kDa proteins in the total cell lysates of human gastric carcinoma cell lines KATO III and MKN-45. Using a proteomic approach, we identified these MG7 immunoreactive proteins as the human heterogeneous nuclear ribonucleoprotein A2/B1 (hnRNP A2/B1). Western blot analysis of nuclear and cytosolic fraction of KATO III cells using either MG7 or hnRNP A2/B1 antibodies confirmed that the target antigen is located exclusively in the nucleus. With the use of archival samples, we also found that the level of hnRNP A2/B1 protein was increased in gastric cancer tissues (4 out of 5 patients), when compared to their corresponding matching normal stomach tissue.
URI: http://hdl.handle.net/10397/25305
ISSN: 1615-9853
EISSN: 1615-9861
DOI: 10.1002/pmic.200401159
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