Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/24875
Title: Effect of mixing time on taxoid production using suspension cultures of Taxus chinensis in a centrifugal impeller bioreactor
Authors: Zhong, JJ
Pan, ZW
Wang, ZY
Wu, J 
Chen, F
Takagi, M
Yoshida, T
Keywords: Centrifugal impeller bioreactor
Mixing time
Oxygen transfer
Plant cell culture
Taxoid production
Taxus chinensis
Issue Date: 2002
Publisher: Soc Bioscience Bioengineering Japan
Source: Journal of bioscience and bioengineering, 2002, v. 94, no. 3, p. 244-250 How to cite?
Journal: Journal of Bioscience and Bioengineering 
Abstract: The effects of fluid mixing on the cell growth and secondary metabolite production of plant cells were investigated in a low-shear centrifugal impeller bioreactor (CIB) system. Suspension cultures of Taxus chinensis cells producing taxuyunnanine C (Tc), a physiologically active secondary metabolite, were used as a model system for this investigation. The mixing time (tm) and volumetric oxygen transfer coefficient (kLa) in the bioreactor were characterized at various cell densities and operating conditions. A constant tm of 5 s or 10 s was maintained during cultivation by adjusting the impeller agitation speed with no detrimental effect on the cultured cells. A higher cell density, Tc content and total Tc production were obtained under the shorter mixing time of 5 s. The favorable effect of more rapid mixing on Tc production was also confirmed when the Tc accumulation was significantly increased through culture elicitation using 100 μM methyl jasmonate (MJA). The lower Tc production at the longer tm of 10 s was mainly attributed to oxygen transfer limitation in the dead zones and larger cell aggregates resulting from poor mixing.
URI: http://hdl.handle.net/10397/24875
ISSN: 1389-1723
DOI: 10.1263/jbb.94.244
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