Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/24239
Title: Protection against hydrogen peroxide-induced injury by Z-ligustilide in PC12 cells
Authors: Yu, Y
Du, JR
Wang, CY
Qian, ZM
Keywords: Cell apoptosis
Neuroprotection
Oxidative injury
Phthalide derivatives
Z-ligustilide
Issue Date: 2008
Publisher: Springer
Source: Experimental brain research, 2008, v. 184, no. 3, p. 307-312 How to cite?
Journal: Experimental brain research 
Abstract: Z-ligustilide (Z-LIG) is the primary lipophilic compound of the Chinese medicine Danggui (Radix Angelica sinensis). Previous studies demonstrated that Z-LIG had significant neuroprotective potential in both transient and permanent cerebral ischemia, possibly through antioxidant and anti-apoptotic mechanisms. The present study examined the mechanisms of Z-LIG on hydrogen peroxide (H 2O2)-induced injury in PC12 cells. Following exposure of the cells to H2O2 (500 μM), a significant reduction in cell survival and total antioxidant capacity (TAC), as well as increased intracellular reactive oxygen species (ROS), were observed. In addition, H 2O2 treatment significantly upregulated Bax expression, cleaved-caspase 3, and cytosolic cytochrome-c, and decreased Bcl-2 protein levels. Pretreatment of the cells with Z-LIG (0.1, 1.0, 2.5, or 5.0 μg/ml) significantly attenuated H2O2-induced cell death, attenuated increased intracellular ROS levels, and decreased Bax expression, cleaved-caspase 3, and cytochrome-c. Further, Z-LIG improved cellular TAC and concentration-dependently upregulated Bcl-2 expression. These results demonstrate that Z-LIG has a pronounced protective effect against H 2O2-induced cytotoxicity, at least partly through improving cellular antioxidant defense and inhibiting the mitochondrial apoptotic pathway. These findings suggest that Z-LIG may be useful in the treatment of neurodegenerative disorders in which oxidative stress and apoptosis are mainly implicated.
URI: http://hdl.handle.net/10397/24239
ISSN: 0014-4819
EISSN: 1432-1106
DOI: 10.1007/s00221-007-1100-3
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