Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/24010
Title: High-throughput determination of biochemical oxygen demand (BOD) by a microplate-based biosensor
Authors: Pang, HL
Kwok, NY
Chan, PH 
Yeung, CH
Lo, W 
Wong, KY 
Issue Date: 2007
Publisher: American Chemical Society
Source: Environmental science & technology, 2007, v. 41, no. 11, p. 4038-4044 How to cite?
Journal: Environmental science & technology 
Abstract: The use of the conventional 5-day biochemical oxygen demand (BOD 5) method in BOD determination is greatly hampered by its time-consuming sampling procedure and its technical difficulty in the handling of a large pool of wastewater samples. Thus, it is highly desirable to develop a fast and high-throughput biosensor for BOD measurements. This paper describes the construction of a microplate-based biosensor consisting of an organically modified silica (ORMOSIL) oxygen sensing film for high-throughput determination of BOD in wastewater. The ORMOSIL oxygen sensing film was prepared by reacting tetramethoxysilane with dimethyldimethoxysilane in the presence of the oxygen-sensitive dye tris(4,7-diphenyl-1,10-phenanthroline)ruthenium-(II) chloride. The silica composite formed a homogeneous, crack-free oxygen sensing film on polystyrene microtiter plates with high stability, and the embedded ruthenium dye interacted with the dissolved oxygen in wastewater according to the Stern-Volmer relation. The bacterium Stenotrophomonas maltophilia was loaded into the ORMOSIL/PVA composite (deposited on the top of the oxygen sensing film) and used to metabolize the organic compounds in wastewater. This BOD biosensor was found to be able to determine the BOD values of wastewater samples within 20 min by monitoring the dissolved oxygen concentrations. Moreover, the BOD values determined by the BOD biosensor were in good agreement with those obtained by the conventional BOD5 method.
URI: http://hdl.handle.net/10397/24010
ISSN: 0013-936X
EISSN: 1520-5851
DOI: 10.1021/es070083k
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