Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/23770
Title: Development and validation of an HPLC-DAD method for bis(12)-hupyridone and its application to a pharmacokinetic study
Authors: Yu, H
Li, WM
Cheung, MC
Zuo, Z
Carlier, PR
Gu, ZM
Chan, K
Huang, M
Wang, YT
Han, YF 
Keywords: Alzheimer's disease
Bis(12)-hupyridone
HPLC-DAD
Pharmacokinetics
Issue Date: 2009
Publisher: Elsevier Science Bv
Source: Journal of pharmaceutical and biomedical analysis, 2009, v. 49, no. 2, p. 410-414 How to cite?
Journal: Journal of Pharmaceutical and Biomedical Analysis 
Abstract: A rapid and simple method of high performance liquid chromatography with UV detection for the quantification of bis(12)-hupyridone in rat blood has been developed and validated. Chromatographic separation was carried out in an Agilent Extend C 18 5 ?gm column (length, 250 mm; inner diameter, 4.6 mm) using a mixture of water-acetonitrile-trifluoroacetic acid (81:19:0.04, v/v/v) as the mobile phase at a flow rate of 1 mL/min, with detection at 229 nm. The method used for the bis(12)-hupyridone quantification showed linearity for concentration range of 0.1-7.5 ?gg/mL with r 2 = 0.9991. The limit of detection and quantification of this method were 0.05 ?gg/mL and 0.1 ?gg/mL, respectively. The intra- and inter-day variations of the analysis were less than 4.22% with standard errors less than 13.3%. The developed method was successfully applied to the pharmacokinetic study of bis(12)-hupyridone after intravenous administration of 5 mg/kg and intraperitoneal administration of 10 and 20 mg/kg in rats.
URI: http://hdl.handle.net/10397/23770
ISSN: 0731-7085
DOI: 10.1016/j.jpba.2008.11.013
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