Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/23528
Title: Correlation between the expression of divalent metal transporter 1 and the content of hypoxia-inducible factor-1 in hypoxic HepG2 cells
Authors: Zhu, L
Zhang, L
Ke, Y
Du, F
Yung, W
Yang, L
Qian, ZM
Keywords: Chemical and physical hypoxia
Divalent metal transporter 1 (DMT1)
Human hepatoma HepG2 cells
Hypoxia-inducible factor-1 (HIF-1)
Hypoxia-inducible gene
Hypoxia/re-oxygenation
Issue Date: 2008
Publisher: Blackwell Publishing
Source: Journal of cellular and molecular medicine, 2008, v. 12, no. 2, p. 569-579 How to cite?
Journal: Journal of Cellular and Molecular Medicine 
Abstract: Transferrin and transferrin receptor are two key proteins of iron metabolism that have been identified to be hypoxia-inducible genes. Divalent metal transporter 1 (DMT1) is also a key transporter of iron under physiological conditions. In addition, in the 5′ regulatory region of human DMT1 (between -412 and -570), there are two motifs (CCAAAGTGCTGGG) that are similar to hypoxia-inducible factor-1 (HIF-1) binding sites. It was therefore speculated that DMT1 might also be a hypoxia-inducible gene. We investigated the effects of hypoxia and hypoxia/re-oxygenation on the expression of DMT1 and the content of HIF-1alpha in HepG2 cells. As we expected, a very similar tendency in the responses of the expression of HIF-1α, DMT1+IRE (iron response element) and DMT1-IRE proteins to chemical (CoCl2) or physical hypoxia was observed. A highly significant correlation was found between the expression of DMT1 proteins and the contents of HIF-1 in hypoxic cells. After the cells were exposed to hypoxia and subsequent normoxia, no HIF-1α could be detected and a significant decrease in DMT1+IRE expression (P<0.05), but not in DMT1-IRE protein (versus the hypoxia group), was observed. The findings implied that the HIF-1 pathway might have a role in the regulation of DMT1+IRE expression during hypoxia.
URI: http://hdl.handle.net/10397/23528
DOI: 10.1111/j.1582-4934.2007.00145.x
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