Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/23396
Title: Altered JS-2 expression in colorectal cancers and its clinical pathological relevance
Authors: Lam, AKY
Gopalan, V
Nassiri, MR
Kasim, K
Dissanayake, J
Tang, JCO 
Smith, RA
Keywords: Adenocarcinoma
Adenoma
Colorectum
JS-2 gene
Mucinous
Issue Date: 2011
Publisher: Elsevier Sci Ltd
Source: Molecular oncology, 2011, v. 5, no. 5, p. 475-481 How to cite?
Journal: Molecular Oncology 
Abstract: JS-2 is a novel gene located at 5p15.2 and originally detected in primary oesophageal cancer. There is no study on the role of JS-2 in colorectal cancer. The aim of this study is to determine the gene copy number and expression of JS-2 in a large cohort of patients with colorectal tumours and correlate these to the clinicopathological features of the cancer patients. We evaluated the DNA copy number and mRNA expression of JS-2 in 176 colorectal tissues (116 adenocarcinomas, 30 adenomas and 30 non-neoplastic tissues) using real-time polymerase chain reaction. JS-2 expression was also evaluated in two colorectal cancer cell lines and a benign colorectal cell line. JS-2 amplification was noted in 35% of the colorectal adenocarcinomas. Significant differences in relative expression levels for JS-2 mRNA between different colorectal tissues were noted (p = 0.05). Distal colorectal adenocarcinoma had significantly higher copy number than proximal adenocarcinoma (p = 0.005). The relative expression level of JS-2 was different between colonic and rectal adenocarcinoma (p = 0.007). Mucinous adenocarcinoma showed higher JS-2 expression than non-mucinous adenocarcinoma (p = 0.02). Early T-stage cancers appear to have higher JS-2 copy number and lower expression of JS-2 mRNA than later stage cancers (p = 0.001 and 0.03 respectively). Colorectal cancer cell lines showed lower expression of JS-2 than the benign colorectal cell line. JS-2 copy number change and expression were shown for the first time to be altered in the carcinogenesis of colorectal cancer. In addition, genetic alteration of JS-2 was found to be related to location, pathological subtypes and staging of colorectal cancer.
URI: http://hdl.handle.net/10397/23396
DOI: 10.1016/j.molonc.2011.06.003
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