Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/23079
Title: Adaptive responses of 25-hydroxyvitamin D-3 1-alpha hydroxylase expression to dietary phosphate restriction in young and adult rats
Authors: Lai, WP
Chau, TS
Cheung, PY
Chen, WF
Lo, SCL 
Favus, MJ
Wong, MS 
Keywords: 25-hydroxyvitamin D-3 1-alpha hydroxylase protein
CYP27B mRNA expression
Aging
Low phosphate diet (LPD)
Renal proximal tubule
Issue Date: 2003
Publisher: Elsevier
Source: Biochimica et biophysica acta. Molecular basis of disease, 2003, v. 1639, no. 1, p. 34-42 How to cite?
Journal: Biochimica et biophysica acta. Molecular basis of disease 
Abstract: Regulation of vitamin D metabolism alters with age. The present study is undertaken to investigate if the loss of renal 1,25-dihydroxyvitamin D-3 (1,25(011)(2)D-3) production in response to dietary phosphate (P) restriction in adult rats is due to an alteration in the renal expression of 25-hydroxyvitamin D-3 1-alpha hydroxylase (l-OHase). Young (4-6 weeks old) and adult (12-14 weeks old) male Sprague Dawley rats were fed either normal P (NPD) or low P diet (LPD) for 0-5 days. Basal expression of l-OHase protein was higher in adult rats. Young rats, but not adult rats, significantly increased l-OHase protein and mRNA expressions in response to LPD in a time-dependent manner. To determine if the stability of renal l-OHase protein changes with LPD feeding, young and adult rats fed either NPD or LPD for 5 days were injected intravenously with cycloheximide (CHX), a protein synthesis inhibitor. CHX decreased l-OHase protein expression in young rats fed NPD. However, CHX did not alter l-OHase protein expression in young rats fed LPD nor in adult rats fed either diet. The results indicate that the stability of renal l-OHase protein increased with age and that LPD increased its stability only in young rats.
URI: http://hdl.handle.net/10397/23079
ISSN: 0925-4439
DOI: 10.1016/S0925-4439(03)00123-6
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