Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/22997
Title: A novel fluorescent biosensor for detection of target DNA fragment from the transgene cauliflower mosaic virus 35S promoter
Authors: Qiu, B
zhang, YS
Lin, YB
Lu, YJ
Lin, ZY
Wong, KY 
Chen, GN
Keywords: Berberine
Cauliflower mosaic virus 35S
Fluorescent biosensor
G-quadruplex
Genetically modified organisms
Issue Date: 2013
Publisher: Elsevier
Source: Biosensors and bioelectronics, 2013, v. 41, no. 1, p. 168-171 How to cite?
Journal: Biosensors and bioelectronics 
Abstract: In this paper, we reported a convenient fluorescence method for the detection of genetically modified organisms (GMOs). As it is known that the cauliflower mosaic virus (CaMV) 35S promoter is widely used in most transgenic plants (Schnurr and Guerra, 2000), we thus design a simple method based on the detection of a section target DNA (DNA-T) from the transgene CaMV 35S promoter. In this method, the full-length guanine-rich single-strand sequences were split into fragments (Probe 1 and 2) and each part of the fragment possesses two GGG repeats. In the presence of K+ ion and berberine, if a complementary target DNA of the CaMV 35S promoter was introduced to hybridize with Probe 1 and 2, a G-quadruplex-berberine complex was thus formed and generated a strong fluorescence signal. The generation of fluorescence signal indicates the presence of CaMV 35S promoter. This method is able to identify and quantify Genetically Modified Organisms (GMOs), and it shows wide linear ranges from 5.0×10-9 to 9.0×10-7mol/L with a detection limit of 2.0×10-9mol/L.
URI: http://hdl.handle.net/10397/22997
ISSN: 0956-5663
EISSN: 1873-4235
DOI: 10.1016/j.bios.2012.08.017
Appears in Collections:Journal/Magazine Article

Access
View full-text via PolyU eLinks SFX Query
Show full item record

SCOPUSTM   
Citations

10
Last Week
0
Last month
0
Citations as of Aug 12, 2017

WEB OF SCIENCETM
Citations

9
Last Week
0
Last month
0
Citations as of Aug 12, 2017

Page view(s)

42
Last Week
1
Last month
Checked on Aug 13, 2017

Google ScholarTM

Check

Altmetric



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.