Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/22996
DC FieldValueLanguage
dc.contributorDepartment of Health Technology and Informatics-
dc.creatorSzeto, YT-
dc.creatorLee, AK-
dc.creatorBenzie, IFF-
dc.date.accessioned2014-12-19T07:06:06Z-
dc.date.available2014-12-19T07:06:06Z-
dc.identifier.issn0960-3271-
dc.identifier.urihttp://hdl.handle.net/10397/22996-
dc.language.isoenen_US
dc.subjectAntioxidanten_US
dc.subjectbuccalen_US
dc.subjectgenoprotectionen_US
dc.subjectlymphocyteen_US
dc.subjectquercetinen_US
dc.titleOptimized noninvasive procedures to measure DNA damage in comet assayen_US
dc.typeJournal/Magazine Articleen_US
dc.identifier.spage1144-
dc.identifier.epage1150-
dc.identifier.volume31-
dc.identifier.issue11-
dc.identifier.doi10.1177/0960327112446816-
dcterms.abstractThe comet assay is a well-established, simple and sensitive method to measure DNA damage in single cell and is commonly used in human trials to investigate the effects of pollution, occupational hazards and potential genoprotective agents. Peripheral blood lymphocytes are most commonly used in human biomonitoring studies, but lymphocytes collected from the mouth offer a potentially attractive, noninvasive alternative. The aim of the current study was to develop a buccal cell lymphocyte comet assay procedure. Cells were collected from mouthwash of three healthy volunteers and tested individually. The comet assay was performed under different pH and times of alkaline treatment, electrophoresis run times and hydrogen peroxide concentrations. Optimal conditions for buccal lymphocytes in comet assay were found to be pH >13 for unwinding and electrophoresis buffers, 10-min alkaline unwinding treatment and 20-min electrophoresis run time. We successfully utilized our optimized assay conditions to demonstrate the genoprotective activity of quercetin. This newly established procedure offers an alternative noninvasive sampling method for the investigation of DNA protection and/or damaging effect.-
dcterms.bibliographicCitationHuman and experimental toxicology, 2012, v. 31, no. 11, p. 1144-1150-
dcterms.isPartOfHuman and Experimental Toxicology-
dcterms.issued2012-
dc.identifier.isiWOS:000310884800006-
dc.identifier.scopus2-s2.0-84869005975-
dc.identifier.pmid22653686-
dc.identifier.rosgroupidr63770-
dc.description.ros2012-2013 > Academic research: refereed > Publication in refereed journal-
Appears in Collections:Journal/Magazine Article
Access
View full-text via PolyU eLinks SFX Query
Show simple item record

SCOPUSTM   
Citations

5
Last Week
0
Last month
0
Citations as of Sep 2, 2020

WEB OF SCIENCETM
Citations

5
Last Week
0
Last month
0
Citations as of Oct 27, 2020

Page view(s)

146
Last Week
2
Last month
Citations as of Oct 25, 2020

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.