Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/22567
Title: Bioconversion of beetle-killed lodgepole pine using SPORL : process scale-up design, lignin coproduct, and high solids fermentation without detoxification
Authors: Zhou, H
Zhu, JY
Luo, X
Leu, SY
Wu, X
Gleisner, R
Dien, BS
Hector, RE
Yang, D
Qiu, X
Horn, E
Negron, J
Issue Date: 2013
Publisher: American Chemical Society
Source: Industrial and engineering chemistry research, 2013, v. 52, no. 45, p. 16057-16065 How to cite?
Journal: Industrial and engineering chemistry research 
Abstract: Mountain pine beetle killed Lodgepole pine (Pinus contorta Douglas ex Loudon) wood chips were pretreated using an acidic sulfite solution of approximately pH = 2.0 at a liquor to wood ratio of 3 and sodium bisulfite loading of 8 wt % on wood. The combined hydrolysis factor (CHF), formulated from reaction kinetics, was used to design a scale-up pretreatment on 2000 g wood chips at a relatively low temperature of 165 C that reduced furan formation and facilitated high solids saccharification and fermentation. The pretreated solids and liquor were disk milled together to result in a biomass whole slurry of 25% total solids. The whole biomass slurry was directly used to conduct simultaneous enzymatic saccharification and combined fermentation (SSCombF) using a commercial cellulase and Saccharomyces cerevisiae YRH400 without detoxification. A terminal ethanol titer of 47.1 g L-1 with a yield of 306 L (tonne wood)-1, or 72.0% theoretical, was achieved when SSCombF was conducted at an unwashed solids loading of 18%. The lignosulfonate (LS) from SPORL was highly sulfonated and showed better dispersibility than a high purity commercial softwood LS, and therefore has potential as a directly marketable coproduct.
URI: http://hdl.handle.net/10397/22567
ISSN: 0888-5885
EISSN: 1520-5045
DOI: 10.1021/ie402873y
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