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Title: Comparison of vascular relaxation, lipolysis and glucose uptake by peroxisome proliferator-activated receptor-gamma activation in +db/+m and +db/+db mice
Authors: Seto, SW
Lam, TY
Leung, GPH
Ngai, SM
Chan, SW
Kwan, YW
Keywords: Peroxisome proliferator-activated receptor-gamma
Aortic relaxation
Glucose uptake
+db/ plus db mice
Issue Date: 2007
Publisher: Elsevier
Source: European journal of pharmacology, 2007, v. 572, no. 1, p. 40-48 How to cite?
Journal: European journal of pharmacology 
Abstract: In this study, we determined the in vitro effect of peroxisome proliferator-activated receptor-gamma (PPAR-gamma) activation on the aortic relaxation, lipolysis and insulin-induced [H-3]-glucose uptake of the abdominal (omental) adipocytes of the non-diabetic (+db/+m) and obese/diabetic (+db/+db) mice. The expression of PPAR-gamma (mRNA and protein) in aorta and adipose tissues was evaluated and compared. Cumulative application of ciglitazone, pioglitazone and troglitazone (PPAR-gamma agonists) caused a concentration-dependent aortic relaxation (sensitive to 2-chloro-5-nitro-N-phenylbenzamide (GW9662) (1 mu M, a selective PPAR-gamma antagonist) and N-omega-nitro-L-arginine methyl ester (L-NAME) (20 mu M, a nitric oxide synthase inhibitor)) with a maximum relaxation of similar to 30% (3 mu M) in +db/+m mice, whereas no relaxation was observed in +db/+db mice. All PPAR-gamma agonists examined did not alter the basal lipolysis of both species, but forskolin caused a concentration-dependent lipolysis, with a greater magnitude observed in +db/+m mice. Insulin (0.1 and 1 mu M) caused an enhancement of [H-3]-glucose uptake into adipocytes with a greater magnitude in +db/+m mice. In contrast, none of the PPAR-gamma agonists tested (0.1, 1 and 10 mu M) altered the basal and the insulin (0.1 mu M)-induced [H-3]-glucose uptake into adipocytes of both species. In addition, there was no difference in PPAR-gamma expression (mRNA and protein) in the aorta and adipose tissues between the species. In conclusion, our results demonstrate that PPAR-gamma is present in the abdominal (omental) adipose tissue and thoracic aorta. An acute activation of PPAR-gamma produced a small (similar to 30%) aortic relaxation (nitric oxide/endothelium-dependent) of +db/+m mice. However, all PPAR-gamma agonists examined have no acute effect on lipolysis and the insulin-induced glucose uptake into adipocytes of both +db/+m and +db/+db mice.
ISSN: 0014-2999
DOI: 10.1016/j.ejphar.2007.05.070
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