Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/21839
Title: A novel method to prepare chitosan powder and its application in cellulase immobilization
Authors: Mao, X
Guo, G
Huang, J
Du, Z
Huang, Z
Ma, L
Li, P 
Gu, L
Keywords: Ammonium sulfate
Cellulase
Chitosan
Immobilized
Microsphere
Sponge
Issue Date: 2006
Source: Journal of chemical technology and biotechnology, 2006, v. 81, no. 2, p. 189-195 How to cite?
Journal: Journal of Chemical Technology and Biotechnology 
Abstract: In this study, chitosan microspheres and sponges with uniform spherical and porous morphologies were prepared by coiling the stretched chains of chitosan with addition of salt and choosing different kinds of organic solvents as evaporation solvents. Cellulase was immobilized to the support by a covalent method. The enzyme exhibited a considerable affinity to the support, and the protein loading of 145.5 mg g-1 support was fairly high. The immobilized cellulase had a higher Km than free cellulase and had better stability with respect to pH, thermal stability, reuses and storage stability than free cellulase.
URI: http://hdl.handle.net/10397/21839
ISSN: 0268-2575
DOI: 10.1002/jctb.1378
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