Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/21722
Title: Glutamate-induced retinal lipid and protein damage : the protective effects of catechin
Authors: Siu, AW
Lau, MK
Cheng, JS
Chow, CK
Tam, WC
Li, KK
Lee, DKL
To, TSS 
To, CH 
Do, CW 
Keywords: Catechin
Glutamate
Lipid
Oxidation
Protein
Retina
Issue Date: 2008
Publisher: Elsevier
Source: Neuroscience letters, 2008, v. 432, no. 3, p. 193-197 How to cite?
Journal: Neuroscience letters 
Abstract: Glutamate toxicity has been implicated in various retinal diseases. Green tea leaf extract catechin has protective effects against cellular toxicity. This study investigated the effects of catechin on the glutamate-treated retina. Porcine retinal homogenates were incubated with glutamate (20 nmol) at 37 °C for 60 min. Catechin was co-incubated with the glutamate-treated retina in the same condition. The malondialdehyde (MDA) levels were determined as an index of lipid peroxidation (LPO). Differential protein expressions were derived from two-dimensional gel electrophoresis. Mass spectrometry was conducted to identify the proteins. Glutamate increased the retinal MDA (p < 0.0001) and catechin reversed the effect (p < 0.0001). There were significant changes in seven proteins after the glutamate treatment (p < 0.05), namely, heterogeneous ribonucleoprotein, thioredoxin peroxidase, 5-hydroxytryptamine receptor, pyruvate dehydrogenase, ARHA protein, peroxiredoxin 6 and proteasome. Catechin significantly reversed the changes in thioredoxin peroxidase, 5-hydroxytryptamine receptor, peroxiredoxin 6 and pyruvate dehydrogenase (p < 0.05). Our study shows that (a) retinal glutamate toxicity is mediated by LPO and protein modification, and (b) catechin ameliorates the toxicity.
URI: http://hdl.handle.net/10397/21722
ISSN: 0304-3940
EISSN: 1872-7972
DOI: 10.1016/j.neulet.2007.12.046
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