Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/21546
Title: Purification of selenium-containing allophycocyanin from selenium-enriched Spirulina platensis and its hepatoprotective effect against t-BOOH-induced apoptosis
Authors: Fan, C
Jiang, J
Yin, X
Wong, KH 
Zheng, W
Chen, T
Keywords: Antioxidant
Apoptosis
Hepatoprotective effect
Selenium-containing allophycocyanin
Spirulina platensis
Issue Date: 2012
Publisher: Elsevier Sci Ltd
Source: Food chemistry, 2012, v. 134, no. 1, p. 253-261 How to cite?
Journal: Food Chemistry 
Abstract: A fast protein liquid chromatographic method for purification of selenium-containing allophycocyanin (Se-APC) from selenium-enriched Spirulina platensis was demonstrated in this study. The purification procedures included ammonium sulphate precipitation and hydroxylapatite chromatography. Highly pure Se-APC with Se concentration of 415.7 μg g -1 protein was finally obtained. In haemolysis assay, increase in MDA content and ROS generation were found in erythrocytes exposed to t-BOOH. Se-APC exhibited higher antioxidant activity and better protective effect than APC on erythrocytes against t-BOOH-induced oxidative damage. Morphological change of erythrocytes as detected by atomic force microscopy was effectively reversed by Se-APC. In the hepatoprotective model, cytotoxicity induced by t-BOOH in HepG2 cells was significantly attenuated by Se-APC. The studies on the mechanism revealed that Se-APC could block the t-BOOH-induced apoptosis and G2/M cell cycle arrest. The findings suggest that Se-APC is a promising seleno-protein with potential applications in treatment of diseases related with oxidative stress.
URI: http://hdl.handle.net/10397/21546
DOI: 10.1016/j.foodchem.2012.02.130
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