Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/21533
DC FieldValueLanguage
dc.contributorDepartment of Health Technology and Informatics-
dc.creatorChan, WS-
dc.creatorTang, BSF-
dc.creatorBoost, MV-
dc.creatorChow, C-
dc.creatorLeung, PHM-
dc.date.accessioned2015-07-14T01:32:54Z-
dc.date.available2015-07-14T01:32:54Z-
dc.identifier.issn0956-5663-
dc.identifier.urihttp://hdl.handle.net/10397/21533-
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.subjectColorimetric assayen_US
dc.subjectGold nanoparticleen_US
dc.subjectMethicillin-resistant Staphylococcus aureusen_US
dc.subjectMRSAen_US
dc.subjectPCRen_US
dc.subjectProbe hybridizationen_US
dc.titleDetection of methicillin-resistant Staphylococcus aureus using a gold nanoparticle-based colourimetric polymerase chain reaction assayen_US
dc.typeJournal/Magazine Articleen_US
dc.identifier.spage105-
dc.identifier.epage111-
dc.identifier.volume53-
dc.identifier.doi10.1016/j.bios.2013.09.027-
dcterms.abstractWe report the use of gold nanoparticles (Au NPs) for direct colourimetric polymerase chain reaction (PCR) detection of methicillin-resistant Staphylococcus aureus (MRSA) in clinical specimens. The colourimetric assay comprised of 2 Au NP probes functionalized with Staphylococcus aureus 23S rRNA- and mecA-specific oligonucleotides. In this study, 72 clinical samples were tested, which included positive blood culture (n=23), urine (n=8), respiratory samples (n=23), as well as wound swabs, pus and body fluid (n=18). Results were recorded qualitatively by direct visual examination and quantitatively by UV-vis spectrophotometry. Using conventional bacterial culture as the gold standard, the sensitivity, specificity, positive predictive value and negative predictive value of this colourimetric assay were 97.14%, 91.89%, 91.89% and 97.14%, respectively, which were comparable to that of commercial real-time PCR assays with a lower cost per reaction. Our assay also showed good agreement with bacterial culture (κ=0.889). The overall detection limit was 500. ng target amplicon, which was comparable to or better than other similar Au NP biosensors. Interestingly, our data revealed the possible relationship between Au NP probe-target hybridization site and assay performance, which might provide hints for design of the Au NP biosensors for nucleic acid detection. To conclude, our study was the first report on the use of Au NP colourimetric assay for direct detection of MRSA in various types of clinical specimens. Further evaluation of the assay is needed in large-scale trials which can also allow for some modifications to streamline the procedures for routine use.-
dcterms.bibliographicCitationBiosensors and bioelectronics, 2014, v. 53, p. 105-111-
dcterms.isPartOfBiosensors and bioelectronics-
dcterms.issued2014-
dc.identifier.isiWOS:000329881100017-
dc.identifier.scopus2-s2.0-84885437727-
dc.identifier.eissn1873-4235-
dc.identifier.rosgroupidr70519-
dc.description.ros2013-2014 > Academic research: refereed > Publication in refereed journal-
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