Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/21470
Title: Effect of laser treatment on the attachment and viability of mesenchymal stem cell responses on shape memory NiTi alloy
Authors: Chan, CW
Hussain, I
Waugh, DG
Lawrence, J
Man, HC 
Keywords: Fiber laser
Laser treatment
Mesenchymal stem cell (MSC)
NiTi
Shape memory alloy (SMA)
Issue Date: 2014
Publisher: Elsevier
Source: Materials science and engineering. C, materials for biological applications, 2014, v. 42, p. 254-263 How to cite?
Journal: Materials science and engineering. C, materials for biological applications 
Abstract: The objectives of this study were to investigate the effect of laser-induced surface features on the morphology, attachment and viability of mesenchymal stem cells (MSCs) at different periods of time, and to evaluate the biocompatibility of different zones: laser-melted zone (MZ), heat-affected zone (HAZ) and base metal (BM) in laser-treated NiTi alloy. The surface morphology and composition were studied by scanning electron microscope (SEM) and X-ray photoemission spectroscopy (XPS), respectively. The cell morphology was examined by SEM while the cell counting and viability measurements were done by hemocytometer and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetric assay. The results indicated that the laser-induced surface features, such as surface roughening, presence of anisotropic dendritic pattern and complete surface Ni oxidation were beneficial to improve the biocompatibility of NiTi as evidenced by the highest cell attachment (4 days of culture) and viability (7 days of culture) found in the MZ. The biocompatibility of the MZ was the best, followed by the BM with the HAZ being the worst. The defective and porous oxide layer as well as the coarse grained structure might attribute to the inferior cell attachment (4 days of culture) and viability (7 days of culture) on the HAZ compared with the BM which has similar surface morphology.
URI: http://hdl.handle.net/10397/21470
ISSN: 0928-4931
DOI: 10.1016/j.msec.2014.05.022
Appears in Collections:Journal/Magazine Article

Access
View full-text via PolyU eLinks SFX Query
Show full item record

SCOPUSTM   
Citations

7
Citations as of Aug 10, 2017

WEB OF SCIENCETM
Citations

8
Last Week
0
Last month
0
Citations as of Aug 15, 2017

Page view(s)

40
Last Week
1
Last month
Checked on Aug 13, 2017

Google ScholarTM

Check

Altmetric



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.