Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/20995
Title: Analysis of the nucleoside content of Cordyceps sinensis using the stepwise gradient elution technique of thin-layer chromatography
Authors: Ma, KW
Chau, FT
Wu, JY 
Keywords: Cordyceps sinensis
Nucleoside
Quantitative analysis
Software
Stepwise gradient elution
Thin-layer chromatography
Issue Date: 2004
Source: Chinese journal of chemistry, 2004, v. 22, no. 1, p. 85-91 How to cite?
Journal: Chinese Journal of Chemistry 
Abstract: Nucleoside is the main class of active components in Cordyceps sinensis. Thin-layer chromatography (TLC) is one of the most commonly used methods in pharmacopoeias for analyzing chemical components of herbal medicine. Since the isocratic elution method cannot be applied successfully in TLC analysis for separating all the nucleoside components, the stepwise gradient elution has been developed in this work to separate eight nucleoside standards with success. In this way, quantitative analyses of the samples of Cordyceps sinensis were achieved via the proposed TLC procedure coupled with the scanning densitometric techniques of CAMAG and TLCQA methods for qualitative and quantitative analysis.
URI: http://hdl.handle.net/10397/20995
ISSN: 1001-604X
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