Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/2074
Title: Immunotherapeutic effect of an interleukin-2 immunoconjugate in animal model
Authors: Leung, Kin-luen
Keywords: Hong Kong Polytechnic University -- Dissertations
Interleukin 2 -- Therapeutic use
Antibody-toxin conjugates
Immunotherapy
Issue Date: 2003
Publisher: The Hong Kong Polytechnic University
Abstract: Recombinant antibody-cytokine fusion proteins are immunocytokines that deliver high cytokine concentrations in the targeted tumor microenvironment and thereby effectively stimulate cellular immune responses against human malignancies. Protoon-cogene HER-2/neu is overexpressed in some human breast, ovarian, prostate and lung cancers and associated with poor prognosis. A novel immunocytokine consisting of a recombinant human interleukin-2 (rhIL-2) and a recombinant humanized single chain antibody that combined the VH and VL portions of a murine monoclonal antibody (520C9) specific for human HER-2/neu positive tumors has been developed. The fusion protein H520C9sFv-rhIL-2 in combination with human peripheral blood mononuclear cells effectively inhibited growth and dissemination of subcutaneous xenograft and lung metastases of human HER-2/neu positive ovarian carcinoma in severe combined immunodeficient mice. In addition, histological staining and immunohistochemical analysis of lung metastases in the treatment group of SCID mice with the H520C9sFv-rhIL-2 and human PBM cells demonstrated significant suppression of metastatic tumor growth. A dose dependent response was also demonstrated by the effective inhibition of lung metastases at a dose level of 6 ug H520C9sFv-rhJL-2 instead of 3 ug H520C9sFv-rhIL-2 per injection with 2 x 105 human PBM cells. Such results demonstrated that immunocytokine-directed inter1eukin-2 therapy to tumors sites could be effective in an adjuvant setting for patients with disseminated metastases of human HER-2/neu positive tumors.
Description: xiv, 87 leaves : ill. ; 30 cm.
PolyU Library Call No.: [THS] LG51 .H577M OR 2003 Leung
URI: http://hdl.handle.net/10397/2074
Rights: All rights reserved.
Appears in Collections:Thesis

Files in This Item:
File Description SizeFormat 
E-thesis_Link.htmFor PolyU Users162 BHTMLView/Open
b17330932.pdfFor All Users (Non-printable)11.03 MBAdobe PDFView/Open
Show full item record

Page view(s)

525
Last Week
3
Last month
Checked on May 22, 2016

Download(s)

222
Checked on May 22, 2016

Google ScholarTM

Check



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.