Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/20645
Title: [D-Lys3]-GHRP-6 exhibits pro-autophagic effects on skeletal muscle
Authors: Yu, AP
Pei, XM
Sin, TK
Yip, SP 
Yung, BY 
Chan, LW
Wong, CS 
Siu, PM 
Keywords: Apoptosis
Autophagy
CXCR4
Ghrelin
GHSR antagonist
Skeletal myofibre
Issue Date: 2015
Publisher: Elsevier
Source: Molecular and cellular endocrinology, 2015, v. 401, p. 155-164 How to cite?
Journal: Molecular and cellular endocrinology 
Abstract: [D-Lys3]-GHRP-6 is regarded as a highly selective growth-hormone secretagogue receptor (GHSR) antagonist and has been widely used to investigate the dependency of GHSR-1a signalling mediated by acylated ghrelin. However, [D-Lys3]-GHRP-6 has been reported to influence other cellular processes which are unrelated to GHSR-1a. This study aimed to examine the effects of [D-Lys3]-GHRP-6 on autophagic and apoptotic cellular signalling in skeletal muscle. [D-Lys3]-GHRP-6 enhanced the autophagic signalling demonstrated by the increases in protein abundances of beclin-1 and LC3 II-to-LC3 1 ratio in both normal muscle and doxorubicin-injured muscle. [D-Lys3]-GHRP-6 reduced the activation of muscle apoptosis induced by doxorubicin. No histological abnormalities were observed in the [D-Lys3]-GHRP-6-treated muscle. Intriguingly, the doxorubicin-induced increase in centronucleated muscle fibres was not observed in muscle treated with [D-Lys3]-GHRP-6, suggesting the myoprotective effects of [D-Lys3]-GHRP-6 against doxorubicin injury. The [D-Lys3]-GHRP-6-induced activation of autophagy was found to be abolished by the co-treatment of CXCR4 antagonist, suggesting that the pro-autophagic effects of [D-Lys3]-GHRP-6 might be mediated through CXCR4. In conclusion, [D-Lys3]-GHRP-6 exhibits pro-autophagic effects on skeletal muscle under both normal and doxorubicin-injured conditions.
URI: http://hdl.handle.net/10397/20645
ISSN: 0303-7207
EISSN: 1872-8057
DOI: 10.1016/j.mce.2014.09.031
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