Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/20558
Title: PCR cloning of type II polyhydroxyalkanoate biosynthesis genes from two Pseudomonas strains
Authors: Zhang, G
Hang, X
Green, P
Ho, KP
Chen, GQ
Keywords: PCR cloning
Polyhydroxyalkanoate
Polyhydroxyalkanoic acid
Polyhydroxyalkanoic acid synthase
Pseudomonas
Issue Date: 2001
Publisher: Elsevier Science Bv
Source: FEMS microbiology letters, 2001, v. 198, no. 2, p. 165-170 How to cite?
Journal: FEMS Microbiology Letters 
Abstract: Two polyhydroxyalkanoate synthase genes, phaC1 from Pseudomonas pseudoalcaligenes HBQ06 and phaC2 from Pseudomonas nitroreducens 0802, were cloned using a PCR cloning strategy based on the type II pha loci property of Pseudomonas strains. The complete open reading frames (ORFs) of phaC1 (P. nitroreducens HBQ06) and phaC2 (P. nitroreducens 0802) were identified from the PCR products. Using the sequence information, the complete PHA synthase genes were PCR cloned directly from the genomic DNA and expressed in Escherichia coli as confirmed by Fourier transform-infrared spectroscopy and gas chromatography. The differences between PhaC1 and PhaC2 were analyzed and the two proteins were suggested to contain different functions and evolution history.
URI: http://hdl.handle.net/10397/20558
ISSN: 0378-1097
DOI: 10.1016/S0378-1097(01)00137-9
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