Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/19735
Title: Prevalence and characterisation of CTX-M beta-lactamases amongst Escherichia coli isolates from healthy food animals in China
Authors: Zheng, H
Zeng, Z
Chen, S 
Liu, Y
Yao, Q
Deng, Y
Chen, X
Lv, L
Zhuo, C
Chen, Z
Liu, JH
Keywords: Extended-spectrum beta-lactamases
Food animals
Plasmid
CTX-M
Escherichia coli
Issue Date: 2012
Publisher: Elsevier
Source: International journal of antimicrobial agents, 2012, v. 39, no. 4, p. 305-310 How to cite?
Journal: International journal of antimicrobial agents 
Abstract: The impact of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae of food animal origins on human health has caught considerable attention worldwide. Intestinal Escherichia coli obtained from healthy food animals (pigs, cattle and poultry) in China were tested for the presence of ESBL genes. CTX-M-producing isolates were further characterised by pulsed-field gel electrophoresis (PFGE), phylogenetic grouping, genetic environment analysis, conjugation and plasmid replicon typing. A total of 127 of the 896 E. coli isolates showed reduced susceptibility to cefotaxime (minimal inhibitory concentration >= 2 mu g/mL). bla(CTX-M) genes were detected in 111 of the 127 isolates. The most common CTX-M types were CTX-M-14 (n = 40), CTX-M-55 (n = 29) and CTX-M-65 (n = 22), followed by CTX-M-27, -15, -98, -24, -3, -102 and -104. CMY-2 was detected in two isolates. High clonal diversity was found amongst CTX-M-producing isolates. Insertion sequence ISEcp1 was observed 42 bp upstream of the start codon of all CTX-M-9 group genes, whereas the spacer region between the right inverted repeats and CTX-M-1 group genes varied from 45 bp to 127 bp. Most bla(CTX-M) genes were transferable by conjugation. IncFII, IncI1, IncFIB, IncN and IncA/C replicons were detected in 28, 21, 7, 5 and 1 of the 70 transconjugants carrying bla(CTX-M), respectively. This study demonstrates that commensal E. coli from healthy food animals can be important reservoirs of bla(CTX-M) genes and may contribute to the dissemination and transfer of these beta-lactamase genes throughout China.
URI: http://hdl.handle.net/10397/19735
ISSN: 0924-8579
EISSN: 1872-7913
DOI: 10.1016/j.ijantimicag.2011.12.001
Appears in Collections:Journal/Magazine Article

Access
View full-text via PolyU eLinks SFX Query
Show full item record

SCOPUSTM   
Citations

82
Citations as of Sep 15, 2017

WEB OF SCIENCETM
Citations

78
Last Week
0
Last month
3
Citations as of Sep 21, 2017

Page view(s)

36
Last Week
0
Last month
Checked on Sep 17, 2017

Google ScholarTM

Check

Altmetric



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.