Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/19704
Title: Plasma ascorbic acid : measurement, stability and clinical utility revisited
Authors: Chung, WY
Chung, JKO
Szeto, YT
Tomlinson, B
Benzie, IFF 
Keywords: Ascorbic acid
FRASC
Stability
Issue Date: 2001
Source: Clinical biochemistry, 2001, v. 34, no. 8, p. 623-627 How to cite?
Journal: Clinical Biochemistry 
Abstract: Aims: To compare plasma ascorbic acid results by the colorimetric FRASC (Ferric Reducing/Antioxidant and Ascorbic Acid) assay and a reference HPLC method; to re-examine plasma ascorbic acid stability, and anticoagulant effect. Design and methods: For method comparison, 31 plasma samples were tested by both methods. For stability, matching EDTA, heparin, citrate and fluoride/oxalate plasma, stored under different conditions of time and temperature, was measured. Results: FRASC is an acceptable alternative to HPLC for plasma ascorbic acid: precision, limit of detection and recovery were similar, and results by the two methods were indistinguishable: mean (95% CI) difference:1.8 (-1.1-4.6; n = 31) μmol/L. Ascorbic acid was most stable in heparinized plasma. Marked loss (p < 0.05) in EDTA plasma occurred within 30 min of blood collection. Conclusions: FRASC offers a speedy and reliable alternative to HPLC for plasma ascorbic acid. Heparin is proposed as the anticoagulant of choice; loss of ascorbic acid is rapid in EDTA plasma ex vivo.
URI: http://hdl.handle.net/10397/19704
ISSN: 0009-9120
DOI: 10.1016/S0009-9120(01)00270-3
Appears in Collections:Journal/Magazine Article

Access
View full-text via PolyU eLinks SFX Query
Show full item record

SCOPUSTM   
Citations

69
Last Week
0
Last month
1
Citations as of Aug 20, 2017

WEB OF SCIENCETM
Citations

63
Last Week
0
Last month
1
Citations as of Aug 20, 2017

Page view(s)

46
Last Week
1
Last month
Checked on Aug 21, 2017

Google ScholarTM

Check

Altmetric



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.