Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/19627
Title: Sphaerotilus natans isolated from activated sludge and its production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate)
Authors: Liu, K
Chua, H
Lo, WH 
Lawford, H
Yu, PHF
Keywords: Activated sludge
Copolymer
Poly(3-hydroxybutyrate-co-3-hydroxyvalerate)
Polymer properties
Sphaerotilus natans
Issue Date: 2002
Publisher: Humana Press
Source: Applied biochemistry and biotechnology. Part A, Enzyme engineering and biotechnology, 2002, v. 98-100, p. 1061-1073 How to cite?
Journal: Applied biochemistry and biotechnology 
Abstract: Sphaerotilus natans is a sheathed bacterium existing in the activated sludge of wastewater treatment plants. It is one of the filamentous bacteria causing the bulking and foaming difficulties of activated sludge. Isolating the strain and culturing it in an axenic environment could not only provide the metabolic knowledge of the strains that would be useful in the development of wastewater treatment methods, but also could enable us to gain an understanding of the mechanism by which poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (poly[3-HB-co-3-HV]) is produced by this strain. This article reports the screening and isolation of the strain from the activated sludge using the Nile blue staining method together with Fourier transform infrared analysis. We investigated the ability of the selected strain to produce poly(3-HB-co-3-HV) cop01ymer using glucose and peptone, or by adding valeric acid or sodium propionate as precursor. Proper precursor feeding could dramatically enhance its 3HV content in the copolymer P(3HB-co-3HV). By controlling the different feeding times in fed-batch fermentation, different desired copolymers were obtained with 15, 40, and 70% 3HV mole fraction of the copolymer. Polymer properties were analyzed by gas chromatography, differential scanning calorimetry, thermo-gravimetry, and nuclear magnetic resonance analysis.
URI: http://hdl.handle.net/10397/19627
ISSN: 0273-2289
EISSN: 1559-0291
DOI: 10.1385/ABAB:98-100:1-9:1061
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