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Title: Activation of group iii metabotropic glutamate receptor reduces intracellular calcium in beta-amyloid peptide [31-35]-treated cortical neurons
Authors: Zhao, L
Zhao, ST
Qian, ZM
Zhang, C
Wu, XM
Du, F
Ke, Y
Keywords: A beta[31-35]
Real time fluorescence imaging technique
Primary cortical neurons
Group III mGluRs
Issue Date: 2009
Publisher: Springer
Source: Neurotoxicity research, 2009, v. 16, no. 2, p. 174-183 How to cite?
Journal: Neurotoxicity Research 
Abstract: It is unknown whether amyloid beta-protein 31-35 (A beta[31-35]) has effects similar to A beta[1-40] and A beta[25-35] on the intracellular calcium ([Ca(2+)]i) to induce a disruption of calcium homeostasis. In this study, we investigated the effects of A beta[31-35] on [Ca(2+)]i in primary cultured cortical neurons using real time fluorescence imaging technique and the Ca(2+)-sensitive dye Furo-2/AM. It was found that A beta[31-35] (25 mu M) could induce a significant elevation in [Ca(2+)]i and a decrease in the average latency in the cortical neurons in a dose-dependent manner. To examine whether the activation of group III mGluRs could block the changes in [Ca(2+)]i and protect neurons from apoptosis induced by A beta[31-35], we then investigated the effects of l-serine-O-phosphate (l-SOP) and (R,S)-4-phosphonophenylglycine ((R,S)-PPG), the selective agonists of group III metabotropic glutamate receptors (mGluRs), on [Ca(2+)]i and apoptosis in neurons treated by A beta[31-35]. We demonstrated that l-SOP or (R,S)-PPG (100 mu M) treatment suppresses significantly the elevation of [Ca(2+)]i induced by A beta[31-35] and also induces an almost complete recovery of both the fluorescence intensity and apoptotic cells (%) to the control level in the neurons. These results suggest that A beta[31-35] may be the shortest sequence responsible for the neuronal toxicity of A beta protein and that the neuroprotective role of the activation of group III mGluRs from the apoptosis induced by A beta[31-35] might be partly due to its ability to inhibit the increased calcium influx, which results from A beta[31-35].
ISSN: 1029-8428
DOI: 10.1007/s12640-009-9068-3
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