Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/19337
Title: Structural elucidation of an exopolysaccharide from mycelial fermentation of a tolypocladium sp fungus isolated from wild cordyceps sinensis
Authors: Yan, JK
Li, L
Wang, ZM
Wu, JY 
Keywords: Cordyceps sinensis
Mycelial fermentation
Exopolysaccharide
Fractionation
Molecular structure
Issue Date: 2010
Publisher: Elsevier Sci Ltd
Source: Carbohydrate polymers, 2010, v. 79, no. 1, p. 125-130 How to cite?
Journal: Carbohydrate Polymers 
Abstract: A novel polysaccharide designated EPS-1A with an average molecular weight around 40 kDa was fractionated and purified by anion-exchange and gel-filtration chromatography from the crude exopolysaccharide (EPS) isolated from fermentation broth of Cs-HK1, a Tolypocladium sp. fungus isolated from wild Cordyceps sinensis. The structural characteristics of EPS-1A were determined with various methods (e.g. GC, GC-MS, FT-IR,(1)H NMR and (13)C NMR) and through acid hydrolysis, methylation, periodate-oxidation and Smith degradation. The results suggested that EPS-1A was composed of glucose, mannose and galactose at 15.2:3.6:1.0 M ratio. EPS-1A was a slightly branched polysaccharide and its backbone was composed of (1 -> 6)-alpha-D-glucose residues (similar to 77%) and (1 -> 6)-alpha-D-mannose residues ( similar to 23%). Branching occurred at O-3 position of (1 -> 6)-alpha-D-mannose residues of the backbone with (1 -> 6)-alpha-D-mannose residues and (1 -> 6)-alpha-D-glucose residues, and terminated with beta-D-galactose residues.
URI: http://hdl.handle.net/10397/19337
ISSN: 0144-8617
DOI: 10.1016/j.carbpol.2009.07.047
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