Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/19054
Title: Staining intensity of individual osteons correlated with elastic properties and degrees of mineralization
Authors: Qin, L
Hung, L
Leung, K
Guo, X 
Bumrerraj, S
Katz, L
Keywords: Contact microradiography
Goat
Mineralization
Osteons
Scanning acoustic microscopy
Surface staining
Issue Date: 2001
Publisher: Springer Tokyo
Source: Journal of bone and mineral metabolism, 2001, v. 19, no. 6, p. 359-364 How to cite?
Journal: Journal of Bone and Mineral Metabolism 
Abstract: Surface staining is widely used for histological studies involving undecalcified thick bone sections. Individual osteons, particularly newly formed ones stained with toluidine blue (TB), show various color intensities. We studied the correlations between TB color intensity and the differences in stiffness and degree of mineralization of individual osteons in undecalcified histological sections of goat tibial diaphysis, measured by scanning acoustic microscopy (SAM) and contact microradiography (CMR), respectively. Results showed that all three measurements correlated significantly with each other (r = 0.567 - 0.786; all P ≤ 0.01). The TB surface staining intensity of individual osteons correlated better with the reflection coefficient (stiffness index) measured by SAM (r = 0.713) than with the aluminum step-wedge equivalent thickness measured on CMR micrographs (r = 0.567). The aluminum step-wedge equivalent thickness of individual osteons on CMR correlated slightly better with the SAM reflection coefficient (r = 0.786) than with the TB surface staining intensity (r = 0.713) The results of this study suggest that TB surface staining may be used as a simple method for indicating differences in stiffness and degree of mineralization in individual osteons in comparative histological studies.
URI: http://hdl.handle.net/10397/19054
ISSN: 0914-8779
DOI: 10.1007/s007740170005
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