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Title: Antiangiogenic activity of a concentrated effective microorganism fermentation extract
Authors: Chui, CH
Gambari, R
Lau, FYI
Hau, DKP
Wong, RSM
Cheng, GYM
Kok, SHL
Higa, T
Ke, B
Chan, ASC
Fong, DWF
Tang, JCO 
Issue Date: 2006
Source: International journal of molecular medicine, 2006, v. 18, no. 5, p. 975-979
Abstract: We have previously demonstrated the possible growth inhibitory activity of both first generation of the effective microorganism fermentation extract (EM-X) as well as the second generation (EM-X2) on cancer cell lines in vitro. The possible anti-angiogenic potential of EM-X has not been reported. Herein we show that using the concentrated EM-X, the growth of human umbilical cord endothelial cells (HUCE) was significantly inhibited in vitro. Enzyme linked immunosorbent assay suggested that the concentrated EM-X is able to reduce the level of vascular endothelial growth factor (VEGF) from Hep3B hepatocellular carcinoma (HCC) cells. The conditioned culture medium obtained from the concentrated EM-X incubated Hep3B HCC cells possessed significant antiproliferative effect on the HUCE cells. Moreover, in vivo chick chorioallantoic membrane assay further demon-strated that the concentrated EM-X is able to greatly inhibit the basic fibroblast growth factor induced angiogenesis from chick embryo experiment. We speculate that the anti-cancer potential of this concentrated EM-X involved growth inhibition on cancer cell and antiangiogenic effect on HUCE cells.
Keywords: Chick chorioallantoic membrane
Effective microorganism fermentation extract
Human umbilical cord endothelial cell
Publisher: Spandidos Publications
Journal: International journal of molecular medicine 
ISSN: 1107-3756
EISSN: 1791-244X
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