Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/18413
Title: Morphology and adhesion of mesenchymal stem cells on PLLA, apatite and apatite/collagen surfaces
Authors: Chen, Y
Cho, MR
Mak, AFT
Li, JS
Wang, M
Sun, S
Issue Date: 2008
Publisher: Springer
Source: Journal of materials science : materials in medicine, 2008, v. 19, no. 7, p. 2563-2567 How to cite?
Journal: Journal of materials science : materials in medicine 
Abstract: Biomimetic apatite/collagen composite coating, previously reported particularly with regard to its fabrication, characterization and interaction with osteoblast-like cells, has been investigated in this study to understand the response of human mesenchymal stem cells (hMSC) to such surface. PLLA films and PLLA films with apatite coating were compared with PLLA films with apatite/collagen composite coating. The hMSC morphology in response to such conditions was first observed using fluorescence microscopy. To further understand such cell-material interactions at a molecular level, integrin expression, actin assembly and vinculin-positive focal adhesion plaques were examined. Our results demonstrated that spreading of stem cells on the apatite/collagen composite surface was determined best among the three types of surfaces, followed by the apatite surface and then the PLLA control. Integrin expression on the apatite/collagen surface was higher than those on the apatite surface and PLLA surface. Immunostaining for vinculin and actin suggested that the composite coating on PLLA enhanced the formation of focal adhesion.
URI: http://hdl.handle.net/10397/18413
ISSN: 0957-4530
DOI: 10.1007/s10856-007-3195-2
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