Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/18412
Title: A PDMS microfluidic system with poly(ethylene glycol)/SU-8 based apertures for planar whole cell-patch-clamp recordings
Authors: Xu, B
Liu, Z
Lee, YK
Mak, A
Yang, M 
Keywords: Micromolding
Patch-clamp
Poly(ethylene glycol)
Whole cell current recording
Issue Date: 2011
Publisher: Elsevier
Source: Sensors and actuators. A, Physical, 2011, v. 166, no. 2, p. 219-225 How to cite?
Journal: Sensors and actuators. A, Physical 
Abstract: Poly(dimethylsiloxane) (PDMS) has been used as an attractive material for planar patch-clamp system fabrication. However, the hydrophobic property of PDMS prevents the establishment of a high-resistance seal with cell membrane. Here, we describe the first use of poly(ethylene glycol) (PEG)/SU-8 mixture for patch-clamp partition electrode fabrication. This PEG/SU8 mixture with weight ratio of 1:1 has been proved to have better properties compared with PDMS for cell-patch interface, such as improved mechanical property, permanent hydrophilic surface property, and easiness to achieve high-resistance cell sealing. The PEG/SU-8 partitions with 2 μm apertures were successfully fabricated by employing a two-step process of soft lithography for PDMS mold (thermal curing) and PEG/SU-8 chip (UV light curing). The partitions could be reversibly sealed to the PDMS microfluidic system including electrode solution inlet/outlet, cell manipulation inlet/outlet, cell reservoir, microchannels and microfabricated Ag/AgCl electrodes. The integrated chip was successfully used to trap Hela cells onto 2 μm aperture by a negative pressure. We demonstrated that a planar PDMS patch-clamp system with PEG/SU-8 based cell-patch interface for successful patch-clamp recording on Hela cells.
URI: http://hdl.handle.net/10397/18412
ISSN: 0924-4247
EISSN: 1873-3069
DOI: 10.1016/j.sna.2009.12.010
Appears in Collections:Journal/Magazine Article

Access
View full-text via PolyU eLinks SFX Query
Show full item record

SCOPUSTM   
Citations

7
Last Week
0
Last month
0
Citations as of Oct 9, 2017

WEB OF SCIENCETM
Citations

6
Last Week
0
Last month
0
Citations as of Oct 16, 2017

Page view(s)

56
Last Week
2
Last month
Checked on Oct 15, 2017

Google ScholarTM

Check

Altmetric



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.