Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/17611
Title: Daily changes in the phosphoproteome of the dinoflagellate lingulodinium
Authors: Liu, B
Lo, SCL 
Matton, DP
Lang, BF
Morse, D
Keywords: Dinoflagellate.
Diurnal rhythm
Phosphopeptide enrichment
Phosphoprotein
Two dimensional gel electrophoresis
Issue Date: 2012
Publisher: Elsevier Gmbh, Urban & Fischer Verlag
Source: Protist, 2012, v. 163, no. 5, p. 746-754 How to cite?
Journal: Protist 
Abstract: The dinoflagellate Lingulodinium has a large number of daily rhythms, many of which have no biochemical correlates. We examined the possibility that changes in protein phosphorylation may mediate some of the rhythmic changes by comparing proteins prepared from midday (LD6) and midnight (LD18) cultures. We used two different methods, one a 2D gel protocol in which phosphoproteins were identified after staining with ProQ Diamond, and the other an LC-MS/MS identification of tryptic phosphopeptides that had been purified by TiO 2 chromatography. Two differentially phosphorylated proteins, a light harvesting complex protein and Rad24, were identified using the 2D gel protocol. Six differentially phosphorylated proteins, a polyketide synthase, an uncharacterized transporter, a LIM (actin binding) domain and three RNA binding domain proteins, were identified using the phosphopeptide enrichment protocol. We conclude that changes in protein phosphorylation may underlie some of the rhythmic behavior of Lingulodinium.
URI: http://hdl.handle.net/10397/17611
ISSN: 1434-4610
DOI: 10.1016/j.protis.2011.11.001
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