Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/17328
Title: Trityl-derivatized carbohydrates immobilized on a polystyrene microplate
Authors: Zou, L
Pang, HL
Chan, PH 
Huang, ZS
Gu, LQ
Wong, KY 
Keywords: Carbohydrate array
Lectin
Microplate
Noncovalent
Issue Date: 2008
Source: Carbohydrate research, 2008, v. 343, no. 17, p. 2932-2938 How to cite?
Journal: Carbohydrate Research 
Abstract: Carbohydrate biosensors, including carbohydrate arrays, are attracting increased attention for the comprehensive and high-throughput investigation of protein-carbohydrate interactions. Here, we describe an effective approach to fabricating a robust microplate-based carbohydrate array capable of probing protein binding and screening for inhibitors in a high-throughout manner. This approach involves the derivatization of carbohydrates with a trityl group through an alkyl linker and the immobilization of the trityl-derivatized carbohydrates (mannose and maltose) onto microplates noncovalently to construct carbohydrate arrays. The trityl carbohydrate derivative has very good immobilization efficiency for polystyrene microplates and strong resistance to aqueous washing. The carbohydrate arrays can probe the interactions with the lectin Concanavalin A and screen this protein for the well-known inhibitors methyl α-d-mannopyranoside and methyl α-d-glucopyranoside in a high-throughput manner. The method described in this paper represents a convenient way of fabricating robust noncovalent carbohydrate arrays on microplates and offers a convenient platform for high-throughput drug screening.
URI: http://hdl.handle.net/10397/17328
ISSN: 0008-6215
DOI: 10.1016/j.carres.2008.08.021
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