Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/17225
Title: The effect of 880 nm low level laser energy on human fibroblast cell numbers : a possible role in hypertrophic wound healing
Authors: Webb, C
Dyson, M
Keywords: 880 nm wavelength
Hypertrophic scar
In vitro fibroblasts
Wound healing
Issue Date: 2003
Publisher: Elsevier
Source: Journal of photochemistry and photobiology. B, Biology, 2003, v. 70, no. 1, p. 39-44 How to cite?
Journal: Journal of photochemistry and photobiology. B, Biology 
Abstract: Low level lasers (LLLs) have been shown to induce therapeutic effects in wound healing. However, there have been few LLL studies on burn wounds which may become unsightly, hypertrophic and impair function. Inhibitory effects on the healing of fibrotic wounds, prone to hypertrophy may be expected to reasonably reduce the problems accompanying hypertrophic scarring. The effects of LLL wavelengths and treatment parameters on wound healing cells in vitro often demonstrate meaningful results and without concurrent ethical difficulties of clinical trials. This experiment investigated the effect of an 880 nm, 16 mW GaA1As diode at 2.4 and 4 J/cm2 on cell numbers of two human fibroblast cell lines, derived from hypertrophic scar (HF) and normal dermal explants (NF), respectively. After irradiation by 880 nm LLL, cell numbers were measured utilising methylene blue bioassay and read by the spectrophotometer in the same microculture plates. HF and NF exhibited decreased cell numbers as compared to sham-irradiated controls. HF cell number, after 2.4 J/cm2, was significantly lower on day 5 (P<0.05). The NF cell numbers were significantly lower on day 4 and/or day 5 (P<0.05). The results have implications on hypertrophic wound healing and further studies are required.
URI: http://hdl.handle.net/10397/17225
ISSN: 1011-1344
EISSN: 1873-2682
DOI: 10.1016/S1011-1344(03)00053-8
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