Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/16452
Title: Simple and sensitive electrochemical DNA detection of primer generation-rolling circle amplification
Authors: Lee, DC
Yip, SP 
Lee, TMH 
Keywords: Electrochemical DNA detection
Indium tin oxide electrode
Isothermal DNA amplification
Methylene blue
Probe immobilization-free
Issue Date: 2013
Publisher: Wiley-VCH
Source: Electroanalysis, 2013, v. 25, no. 5, p. 1310-1315 How to cite?
Journal: Electroanalysis 
Abstract: A new electrochemical sequence-specific DNA detection platform based on primer generation-rolling circle amplification (PG-RCA), methylene blue (MB) redox indicator, and indium tin oxide (ITO) electrode is reported. In the presence of a specific target sequence, PG-RCA, an isothermal DNA amplification technique, produced large amounts of amplicons in an exponential manner. In addition to the standard components, the reaction mixture contained MB, which bound with the PG-RCA amplicons. End-point electrochemical measurement by differential pulse voltammetry (DPV) was performed using ITO electrode. The amplicon-bound MB resulted in a lower DPV signal than free MB due to a smaller diffusion coefficient as well as electrostatic repulsion between the negatively charged amplicon-bound MB and ITO electrode. With simple assay design (recognition probe) and instrumentation (operating temperature at 37 °C and ITO electrode without the need for probe immobilization), this detection platform is well suited for point-of-care and on-site testing. Real-time measurement was also achieved by pretreating the ITO electrode with bovine serum albumin.
URI: http://hdl.handle.net/10397/16452
ISSN: 1040-0397
EISSN: 1521-4109
DOI: 10.1002/elan.201300029
Appears in Collections:Journal/Magazine Article

Access
View full-text via PolyU eLinks SFX Query
Show full item record

SCOPUSTM   
Citations

10
Last Week
4
Last month
Citations as of Sep 17, 2017

WEB OF SCIENCETM
Citations

11
Last Week
0
Last month
0
Citations as of Sep 15, 2017

Page view(s)

59
Last Week
1
Last month
Checked on Sep 17, 2017

Google ScholarTM

Check

Altmetric



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.