Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/16161
Title: Photodynamic effect in medulloblastoma : downregulation of matrix metalloproteinases and human telomerase reverse transcriptase expressions
Authors: Chu, ESM
Wong, TKS
Yow, CMN
Issue Date: 2007
Publisher: Royal Soc Chemistry
Source: Photochemical and photobiological sciences, 2007, v. 7, no. 1, p. 76-83 How to cite?
Journal: Photochemical and Photobiological Sciences 
Abstract: Tumor invasion and immortality are the most unfavorable drawbacks after cancer treatment. In this study, we focus on determining the photodynamic modulation of the proteolytic enzymes, matrix metalloproteinases (MMP); and a core catalytic subunit of telomerase, the human telomerase reverse transcriptase (hTERT) in medulloblastoma (MED) cell line (TE-671). Hexvix (ALA-H) mediated photodynamic therapy (PDT) demonstrated greater efficacy than 5-aminolevulinic acid (5-ALA) in terms of drug uptake and anti-proliferative effect. Both MMP-2 and hTERT expression are down-regulated quantitatively using ELISA and reverse-transcriptase-PCR (RT-PCR) respectively at post-treatment for this cell line. The MMP-9 expression remains unchanged after treatment. Further, there is a statistically significant inhibition of cell migration at 24 h post-ALA-H-PDT at LD50 (0.01 mM, 2 J cm-2; p < 0.001) in MED TE-671 cells. Evidently, MMP-2 and hTERT mRNA expressions can be the targets for the photodynamic intervention on tumor cell migration and immortality. Hence, PDT may be an alternate cancer regime for medulloblastoma.
URI: http://hdl.handle.net/10397/16161
ISSN: 1474-905X
DOI: 10.1039/b703417b
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