Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/16109
Title: An automated, specific, spectrophotometric method for measuring ascorbic acid in plasma (EFTSA)
Authors: Benzie, IFF 
Keywords: Antioxidants
Ascorbate oxidase
Ascorbic acid
Dehydroascorbic acid
EFTSA
Nutrition
Oxidative stress
Vitamin C
Issue Date: 1996
Source: Clinical biochemistry, 1996, v. 29, no. 2, p. 111-116 How to cite?
Journal: Clinical Biochemistry 
Abstract: Objective: To evaluate an automated enzyme linked, ferrictripyridyltriazine spectrophotometric assay (EFTSA) for plasma ascorbic acid. Design and Methods: Using aqueous ascorbic acid solutions and plasma containing native and/or added ascorbic acid, the following were assessed: reaction kinetics, dose response relationships, recovery of added ascorbic acid, specificity, precision. Results: Performing the test on a Cobas Fara centrifugal analyser, the test is linear at least to 400 μmol/L; within-run CVs at 20, 50, 140, and 300 μmol/L ascorbic acid, in both pure aqueous solutions and in plasma, were <5.5%; 99-105% of added ascorbic acid (from 30- 130 μmol/L) was recovered. The reaction of ascorbic acid is virtually instantaneous; other native antioxidants do not appear to interfere, and there is no interference by dehydroascorbic acid when readings are taken within a 15-60-s reaction time window. Conclusion: EFTSA appears suitable for the routine measurement of ascorbic acid in plasma.
URI: http://hdl.handle.net/10397/16109
ISSN: 0009-9120
DOI: 10.1016/0009-9120(95)02013-6
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