Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/15750
Title: Structural characterisation and immunomodulatory property of an acidic polysaccharide from mycelial culture of Cordyceps sinensis fungus Cs-HK1
Authors: Wang, ZM
Peng, X
Lee, KLD
Tang, JCO 
Cheung, PCK
Wu, JY 
Keywords: Cordyceps sinensis
Exopolysaccharides
Fractionation
Immuno-activity
Mecylial culture
Structure
Issue Date: 2011
Publisher: Elsevier
Source: Food chemistry, 2011, v. 125, no. 2, p. 637-643 How to cite?
Journal: Food chemistry 
Abstract: An acidic polysaccharide, designated AEPS-1, was fractionated from the exopolysaccharide (EPS) produced by a medicinal fungus Cordyceps sinensis Cs-HK1 in mycelial culture. The molecular structure of AEPS-1 was characterised and elucidated by spectral and chromatographic analyses, and through derivatization by periodate oxidation, Smith degradation and methylation. AEPS-1 was composed of glucopyranose (Glcp) and pyrano-glucuronic acid (GlcUp) in an 8:1. M ratio plus a trace amount of mannose, having an average molecular weight of about 36. kDa. AEPS-1 had a linear backbone of (1→3)-linked α-d-Glcp residues with two branches, α-d-Glcp and α-d-GlcUp, attached to the main chain by (1→6) glycosidic bonds at every seventh α-d-Glcp unit. Atomic force microscopy revealed that AEPS-1 formed large networks in water that are connected primarily with triple helical strands. In Raw264.7 macrophage cell cultures, AEPS-1, at suitable doses between 25 and 250 μg/ml, significantly stimulated the release of several major cytokines, demonstrating an immunomodulatory property.
URI: http://hdl.handle.net/10397/15750
ISSN: 0308-8146
DOI: 10.1016/j.foodchem.2010.09.052
Appears in Collections:Journal/Magazine Article

Access
View full-text via PolyU eLinks SFX Query
Show full item record

SCOPUSTM   
Citations

59
Last Week
0
Last month
3
Citations as of Aug 19, 2017

WEB OF SCIENCETM
Citations

51
Last Week
0
Last month
1
Citations as of Aug 12, 2017

Page view(s)

36
Last Week
1
Last month
Checked on Aug 13, 2017

Google ScholarTM

Check

Altmetric



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.