Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/15717
Title: Seasonal variations in the isoflavonoids of Radix Puerariae
Authors: Chen, S
Yang, D
Chen, S
Xu, H
Chan, ASC
Keywords: Content variation
HPLC
Isoflavonoid
Pueraria lobata
Radix Puerariae
UV-spectrophotometry
Issue Date: 2007
Publisher: Wiley-Blackwell
Source: Phytochemical analysis, 2007, v. 18, no. 3, p. 245-250 How to cite?
Journal: Phytochemical Analysis 
Abstract: The variations in isoflavonoid content in Radix Puerariae, the root of Pueraria lobata (Wild) Ohwi, have been examined in order to determine the optimum time to harvest the herb. Total isoflavonoid contents have been analysed by a UV spectrophotometric method whilst HPLC with photodiode array detection has been used to monitor the contents of seven major individual isoflavonoids, namely, 3??-hydroxypuerarin, puerarin, 3??-methoxypuerarin, daidzin, genistin, formononetin-7-glucoside and daidzein. Ninety-six samples of Radix Puerariae of different ages and harvested in different months were collected and analysed. The results clearly showed that 3-year-old roots harvested in January have the highest yields of isoflavonoid compounds. Moreover, the results obtained by both methods have a strong correlation. This suggested that the simple and fast UV spectrophotometric method could be used for monitoring the phytochemical quality of Radix Puerariae.
URI: http://hdl.handle.net/10397/15717
ISSN: 0958-0344
DOI: 10.1002/pca.978
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