Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/14977
Title: Two-in-one gel for spot matching after two-dimensional electrophoresis
Authors: Wang, YY
Cheung, PY
Wong, MS 
Lo, SCL 
Keywords: Two-in-one gel
Issue Date: 2003
Publisher: Wiley-VCH
Source: Proteomics, 2003, v. 3, no. 5, p. 580-583 How to cite?
Journal: Proteomics 
Abstract: Two-dimensional electrophoresis (2-DE) is one of the most commonly used techniques in proteomic investigations. However, due to the complex interplay of incidence including significant biological sample variations, lengthy steps involved in performing 2-DE as well as exposure time with silver staining, it is sometimes difficult to differentiate authentic differences caused by drug treatment with those artifacts caused by sample variations, running conditions of 2-DE as well as treatment time in silver staining etc. If we can compare pooled samples of control and treatment groups run in a single gel and stained together, we would be more comfortable with our findings. We propose here a low cost and highly effective method for locating differentially expressed proteins before and after drug treatment. This "two-in-one gel" technique might partially solve the problems mentioned above.
URI: http://hdl.handle.net/10397/14977
ISSN: 1615-9853
EISSN: 1615-9861
DOI: 10.1002/pmic.200300423
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