Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/14704
Title: Evaluation of the expression of collagen type I in porous calcium phosphate ceramics implanted in an extra-osseous site
Authors: Qu, SX
Guo, X 
Weng, J
Cheng, JCY
Feng, B
Yeung, HY
Zhang, XD
Keywords: Calcium phosphate
Collagen
Extra-osseous implantation
Heterotopic osteogenesis
Histology
Immunohistochemistry
Issue Date: 2004
Publisher: Elsevier Sci Ltd
Source: Biomaterials, 2004, v. 25, no. 4, p. 659-667 How to cite?
Journal: Biomaterials 
Abstract: The aim of the present study is to demonstrate the newly formed tissue in calcium phosphate (Ca/P) ceramics after extra-osseous implantation by histological and immunohistochemistry (IHC) methods. Synthesis porous Ca/P ceramics without adding any growth factor and living cell were implanted in the dorsal muscle of dogs for 1 and 2 months. Undecalcified and decalcified sections were stained by hematoxylin and eosin (H&E), and IHC, respectively. The histological results showed the beginning of osteogenesis and angiogenesis after being implanted for 1 month and the obvious new bone formation after being implanted for 2 months. IHC were conducted via the avidin-biotin peroxidase complex (ABC) method and the primary antibody was collagen type I. IHC results indicated that collagen type I was expressed within osteoblast-like cells and newly formed bone-like tissue in Ca/P ceramics after 1 month, and in the mineralized matrix of newly formed bone and osteoblasts, some osteocytes and some lacunae after 2 months. No cartilage and chondrocytes were observed in the histological and IHC-stained sections. Evidence of intramembranous osteogenesis was confirmed.
URI: http://hdl.handle.net/10397/14704
ISSN: 0142-9612
DOI: 10.1016/S0142-9612(03)00577-5
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