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Title: Screening of thalassemia in Hong Kong Chinese by HPLC method
Authors: Tse, YK
Yow, CMN
Szeto, YT
Keywords: HbA2 HbF
Reference range
Issue Date: 2001
Source: Turkish journal of haematology, 2001, v. 18, no. 1, p. 27-31 How to cite?
Journal: Turkish Journal of Haematology 
Abstract: The prevalence of α-thalassemia and HbH disease are high in Hong Kong Chinese. The Bio-Rad VARIANT, a cation exchange HPLC method for HBA2, HbF and certain haemoglobin variants determination provide a rapid screening for thalassemia. In this study, the reference ranges of HbA2 and HbF in Hong Kong Chinese were first established for this analyser. It was 2.2-3.0% and 0-1.0% respectively which are similar to Caucasians. In addition, in agreement with the manufacturer's pilot study, the system was confirmed to be able to detect HbH. We have demonstrated a peak at 0.5 min observed in the chromatogram of all 30 HbH disease specimens. To conclude, HPLC method shows a similar reference range of HbA2 and HbF between the Hong Kong Chinese and the Caucasians. It also provides a rapid diagnosis of HbH disease which is common in south-east Asia. Manual determination of HbA2 using Bio-Rad Microcolumn HbA2 kit and Betke's manual method for HbF were comparable with the Bio-Rad VARIANT.
ISSN: 1300-7777
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