Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/13808
Title: Mapping live cell viscosity with an aggregation-induced emission fluorogen by means of two-photon fluorescence lifetime imaging
Authors: Chen, S
Hong, Y
Zeng, Y
Sun, Q
Liu, Y
Zhao, E
Bai, G
Qu, J
Hao, J 
Tang, BZ
Keywords: Aggregation
Fluorescent probes
Imaging agents
Microviscosity
Sensors
Issue Date: 2015
Publisher: Wiley-VCH Verlag
Source: Chemistry - A European journal, 2015, v. 21, no. 11, p. 4315-4320 How to cite?
Journal: Chemistry - A European Journal 
Abstract: Intracellular viscosity is a crucial parameter that indicates the functioning of cells. In this work, we demonstrate the utility of TPE-Cy, a cell-permeable dye with aggregation-induced emission (AIE) property, in mapping the viscosity inside live cells. Owing to the AIE characteristics, both the fluorescence intensity and lifetime of this dye are increased along with an increase in viscosity. Fluorescence lifetime imaging of live cells stained with TPE-Cy reveals that the lifetime in lipid droplets is much shorter than that from the general cytoplasmic region. The loose packing of the lipids in a lipid droplet results in low viscosity and thus shorter lifetime of TPE-Cy in this region. It demonstrates that the AIE dye could provide good resolution in intracellular viscosity sensing. This is also the first work in which AIE molecules are applied in fluorescence lifetime imaging and intracellular viscosity sensing.
URI: http://hdl.handle.net/10397/13808
ISSN: 0947-6539
DOI: 10.1002/chem.201405658
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