Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/13808
Title: Mapping live cell viscosity with an aggregation-induced emission fluorogen by means of two-photon fluorescence lifetime imaging
Authors: Chen, S
Hong, Y
Zeng, Y
Sun, Q
Liu, Y
Zhao, E
Bai, G
Qu, J
Hao, J 
Tang, BZ
Issue Date: 2015
Source: Chemistry - a European journal, 2015, v. 21, no. 11, p. 4315-4320
Abstract: Intracellular viscosity is a crucial parameter that indicates the functioning of cells. In this work, we demonstrate the utility of TPE-Cy, a cell-permeable dye with aggregation-induced emission (AIE) property, in mapping the viscosity inside live cells. Owing to the AIE characteristics, both the fluorescence intensity and lifetime of this dye are increased along with an increase in viscosity. Fluorescence lifetime imaging of live cells stained with TPE-Cy reveals that the lifetime in lipid droplets is much shorter than that from the general cytoplasmic region. The loose packing of the lipids in a lipid droplet results in low viscosity and thus shorter lifetime of TPE-Cy in this region. It demonstrates that the AIE dye could provide good resolution in intracellular viscosity sensing. This is also the first work in which AIE molecules are applied in fluorescence lifetime imaging and intracellular viscosity sensing.
Keywords: Aggregation
Fluorescent probes
Imaging agents
Microviscosity
Sensors
Publisher: Wiley-VCH
Journal: Chemistry - a European journal 
ISSN: 0947-6539
EISSN: 1521-3765
DOI: 10.1002/chem.201405658
Appears in Collections:Journal/Magazine Article

Access
View full-text via PolyU eLinks SFX Query
Show full item record

SCOPUSTM   
Citations

50
Last Week
0
Last month
0
Citations as of Sep 8, 2020

WEB OF SCIENCETM
Citations

49
Last Week
0
Last month
1
Citations as of Sep 27, 2020

Page view(s)

213
Last Week
5
Last month
Citations as of Sep 21, 2020

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.