Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/13268
Title: Metagenomic analysis reveals potential biodegradation pathways of persistent pesticides in freshwater and marine sediments
Authors: Fang, H
Cai, L
Yang, Y
Ju, F
Li, X 
Yu, Y
Zhang, T
Keywords: Biodegradation pathway
DDT
HCH
High-throughput sequencing
Pesticide
Sediment
Issue Date: 2014
Publisher: Elsevier
Source: Science of the total environment, 2014, v. 470-471, p. 983-992 How to cite?
Journal: Science of the total environment 
Abstract: The abundance and diversity of biodegradation genes (BDGs) and potential degradation pathways of dichlorodiphenyltrichloroethane (DDT), hexachlorocyclohexane (HCH), and atrazine (ATZ) in freshwater and marine sediments were investigated by metagenomic analysis using 6 datasets (16. Gb in total). The datasets were derived using Illumina high-throughput sequencing and were based on BLAST against self-established databases of BDGs, DDT degradation genes (DDGs), HCH degradation genes (HDGs), and ATZ degradation genes (ADGs). The results showed that the abundance and diversity of BDGs, DDGs, HDGs, and ADGs varied with sample source and locations. The lip and mnp genes, which encode for peroxidase, and the carA gene, which encodes for laccase, were detected as the dominant genes for degradation of organic pollutants. The hdt, hdg, and atzB genes, which encode for hydratase, dehalogenase, and ethylaminohydrolase, were found to be the most abundant genes involved in DDT, HCH, and ATZ degradation, respectively. The identified 69 genera capable of degrading organic pollutants were mostly affiliated with Proteobacteria (49.3%) and Actinobacteria (21.7%). Four genera, including Plesiocystis, Anaerolinea, Jannaschia, and Mycobacterium, were the major biodegradation populations in all sediments. In this study, the nearly complete biodegradation pathways of DDT and ATZ were found, and the partial degradation pathway of HCH was detected in all sediments.
URI: http://hdl.handle.net/10397/13268
ISSN: 0048-9697
EISSN: 1879-1026
DOI: 10.1016/j.scitotenv.2013.10.076
Appears in Collections:Journal/Magazine Article

Access
View full-text via PolyU eLinks SFX Query
Show full item record

SCOPUSTM   
Citations

24
Last Week
1
Last month
1
Citations as of Sep 19, 2017

WEB OF SCIENCETM
Citations

22
Last Week
0
Last month
2
Citations as of Sep 14, 2017

Page view(s)

57
Last Week
1
Last month
Checked on Sep 18, 2017

Google ScholarTM

Check

Altmetric



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.