Please use this identifier to cite or link to this item:
http://hdl.handle.net/10397/12973
DC Field | Value | Language |
---|---|---|
dc.contributor | Department of Applied Biology and Chemical Technology | - |
dc.creator | Li, RWS | en_US |
dc.creator | Yang, C | en_US |
dc.creator | Kwan, YW | en_US |
dc.creator | Chan, SW | en_US |
dc.creator | Lee, SMY | en_US |
dc.creator | Leung, GPH | en_US |
dc.date.accessioned | 2015-06-23T09:16:26Z | - |
dc.date.available | 2015-06-23T09:16:26Z | - |
dc.identifier.issn | 1663-9812 | en_US |
dc.identifier.uri | http://hdl.handle.net/10397/12973 | - |
dc.language.iso | en | en_US |
dc.publisher | Frontiers Research Foundation | en_US |
dc.rights | Copyright: © 2013 Li, Yang, Kwan, Chan, Lee and Leung. This is an open-access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/3.0/), which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc. | en_US |
dc.rights | The following publication Li RWS, Yang C, Kwan YW, Chan SW, Lee SMY and Leung GPH (2013) Involvement of organic cation transporter-3 and plasma membrane monoamine transporter in serotonin uptake in human brain vascular smooth muscle cells. Front. Pharmacol. 4:14,1-8 is available at https://dx.doi.org/10.3389/fphar.2013.00014 | en_US |
dc.subject | Monoamine transporter | en_US |
dc.subject | Organic cation transporter | en_US |
dc.subject | Serotonin | en_US |
dc.subject | Vascular smooth muscle cells | en_US |
dc.title | Involvement of organic cation transporter-3 and plasma membrane monoamine transporter in serotonin uptake in human brain vascular smooth muscle cells | en_US |
dc.type | Journal/Magazine Article | en_US |
dc.identifier.spage | 1 | en_US |
dc.identifier.epage | 8 | en_US |
dc.identifier.volume | 4 | en_US |
dc.identifier.doi | 10.3389/fphar.2013.00014 | en_US |
dcterms.abstract | The serotonin (5-HT) uptake system is supposed to play a crucial part in vascular functions by "fine-tuning" the local concentration of 5-HT in the vicinity of 5-HT2 receptors in vascular smooth muscle cells. In this study, the mechanism of 5-HT uptake in human brain vascular smooth muscle cells (HBVSMCs) was investigated. [3H]5-HT uptake in HBVSMCs was Na+-independent. Kinetic analyses of [3H]5-HT uptake in HBVSMCs revealed a Km of 50.36 ± 10.2 mM and a Vmax of 1033.61 ± 98.86 pmol/mg protein/min. The specific serotonin re-uptake transporter (SERT) inhibitor citalopram, the specific norepinephrine transporter (NET) inhibitor desipramine, and the dopamine transporter (DAT) inhibitor GBR12935 inhibited 5-HT uptake in HBVSMCs with IC50 values of 97.03 ± 40.10, 10.49 ± 5.98, and 2.80 ± 1.04 μM, respectively. These IC50 values were 100-fold higher than data reported by other authors, suggesting that those inhibitors were not blocking their corresponding transporters. Reverse transcription-polymerase chain reaction results demonstrated the presence of mRNA for organic cation transporter (OCT)-3 and plasma membrane monoamine transporter (PMAT), but the absence of OCT-1, OCT-2, SERT, NET, and DAT. siRNA knockdown of OCT-3 and PMAT specifically attenuated 5-HT uptake in HBVSMCs. It is concluded that 5-HT uptake in HBVSMCs was mediated predominantly by a low-affinity and Na+-independent mechanism. The most probable candidates are OCT-3 and PMAT, but not the SERT. | - |
dcterms.accessRights | open access | en_US |
dcterms.bibliographicCitation | Frontiers in pharmacology, 12 Feb. 2013, v. 4, 14, p. 1-8 | en_US |
dcterms.isPartOf | Frontiers in Pharmacology | en_US |
dcterms.issued | 2013-02-12 | - |
dc.identifier.scopus | 2-s2.0-84881492556 | - |
dc.identifier.pmid | 23407616 | - |
dc.identifier.artn | 14 | en_US |
dc.identifier.rosgroupid | r66570 | - |
dc.description.ros | 2012-2013 > Academic research: refereed > Publication in refereed journal | en_US |
dc.description.oa | Version of Record | en_US |
dc.identifier.FolderNumber | OA_IR/PIRA | en_US |
dc.description.pubStatus | Published | en_US |
Appears in Collections: | Journal/Magazine Article |
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File | Description | Size | Format | |
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Li_Organic_Cation_Transporter-3.pdf | 1.18 MB | Adobe PDF | View/Open |
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